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KMID : 0894520080120010107
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Development & Reproduction
2008 Volume.12 No. 1 p.107 ~ p.112
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Production of C21-Steroids in Longchin Goby, Chasmichthys dolichognathus during Oocyte Maturation
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Baek Hea-Ja
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Abstract
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To investigate the C₂₁-steroids produced from maturating oocytes in the longchin goby, Chasmichthys dolichognathus, the oocytes (0.74~0.97 mm) were incubated with radiolabeled 17α-hydroxyprogesterone (³H-17α OHP) for 24 hours. The resulting metabolites were analyzed by thin layer chromatography and identified by gas chromatography-mass spectrometry. Two C₂₁-steroids, 17α-hydroxy,20α-dihydroprogesterone (17α20αP) and 17α-hydroxy,20β-dihydropro-gesterone (17α20βP), were converted from 17αOHP in the maturing oocytes. These two main metabolites were detected at 0.80 mm diameter oocytes or greater. In addition, the effects of these metabolites on in vitro germinal vesicle breakdown (GVBD) were tested. The sensitivity of oocytes to the induction of GVBD was greater at 17α20βP than 17α20αP. This result showed that 17α20βP is a major maturation inducing steroid (MIS) in longchin goby, suggesting 17α20αP may play a role in regulating the oocyte maturation process.
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KEYWORD
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Chasmichthys dolichognathus, Goby, Maturation inducing steroid, C₂₁-steroids, Oocytes, GVBD
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